Human C4b-binding protein beta chain(C4BPB) ELISA kit

Human C4b-binding protein beta chain(C4BPB) ELISA kit

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Mfr.No.

CSB-EL003954HU

Sensitivity

0.78 ng/mL

Detection Range

3.12 ng/mL-200 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

complement component 4 binding protein, beta, C4BP, C4b binding protein, beta chain, complement component 4 binding protein, beta chain, complement component 4-binding protein, beta

Recovery

The recovery of rat OxLDL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-100
EDTA plasma (n=4)	91	89-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat OxLDL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	92
	Range %	87-96
1:200	Average %	94
	Range %	90-99
1:400	Average %	100
	Range %	96-106
1:800	Average %	90
	Range %	85-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.668	2.783	2.726	2.541
200	2.296	2.284	2.290	2.105
100	1.704	1.735	1.720	1.535
50	1.076	1.083	1.080	0.895
25	0.607	0.624	0.616	0.431
12.5	0.408	0.417	0.413	0.228
6.25	0.291	0.298	0.295	0.110
0	0.184	0.186	0.185	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P20851

Applications	ELISA
Research Area	C4BPB

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501573	CSB-EL003954HU	96T	$695	Add to Cart
BHE10501573	CSB-EL003954HU	5x96T	$3336	Add to Cart
BHE10501573	CSB-EL003954HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.