Matrices listed below were spiked with certain level of C4 and the recovery rates were calculated by comparing the measured value to the expected amount of C4 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
97
EDTA plasma(n=5)
87-105
96
heparin plasma(n=5)
87-105
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.