Human C1qC(Complement Component 1, Q Subcomponent C) ELISA Kit

Mfr.No.

ELK3574

Alternative Names

C1QG; Complement Component 1 q subcomponent,Gamma Polypeptide

Reactivity

Human

Detection Range

31.25-2000 pg/mL

Sample Type

Other biological fluids, Serum, Plasma

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant C1qC and the recovery rates were calculated by comparing the measured value to the expected amount of C1qC in samples.

Matrix	Recovery range	Average
serum(n=5)	80-92%	86%
EDTA plasma(n=5)	85-97%	91%
Heparin plasma(n=5)	83-95%	89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C1qC and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	87-96%	85-97%	85-94%	87-96%
EDTA plasma(n=5)	93-104%	83-96%	95-103%	88-95%
Heparin plasma(n=5)	86-98%	93-106%	89-97%	85-94%

Typical Data

<table> <tbody><tr> <th>Concentration (pg/mL)</th> <th>OD</th> <th>Corrected OD</th> </tr> <tr> <td>2000.00</td> <td>2.079</td> <td>2.002</td> </tr> <tr> <td>1000.00</td> <td>1.562</td> <td>1.485</td> </tr> <tr> <td>500.00</td> <td>1.182</td> <td>1.105</td> </tr> <tr> <td>250.00</td> <td>0.897</td> <td>0.820</td> </tr> <tr> <td>125.00</td> <td>0.527</td> <td>0.450</td> </tr> <tr> <td>62.50</td> <td>0.319</td> <td>0.242</td> </tr> <tr> <td>31.25</td> <td>0.100</td> <td>0.023</td> </tr> <tr> <td>0.00</td> <td>0.077</td> <td>0.000</td> </tr> </tbody></table>

Sensitivity

13.7 pg/mL

Limit Of Detection

2000 pg/mL

UniProt ID	P02747
Antigen	C1qC
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C1qC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C1qC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C1qC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C1qC in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Immune molecule;
Protocol	<a href="https://s3.us-west-1.amazonaws.com/s3.biohippo.com/elisakits/elk/ELK3574-1.pdf" target="_blank">Datasheet</a>
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15202804	ELK3574	48T	$320		Add to Cart
BHE15202804	ELK3574	96T	$458		Add to Cart

Human C1qC(Complement Component 1, Q Subcomponent C) ELISA Kit

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