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Home / ELISA Kit

Human C-type mannose receptor 2 (MRC2) ELISA Kit

SKU : BHE13703757

MRC2 encodes a member of the mannose receptor family of proteins that contain a fibronectin type II domain and multiple C-type lectin-like domains. C-type mannose receptor 2 plays a role in extracellular matrix remodeling by mediating the internalization and lysosomal degradation of collagen ligands. Expression of MRC2 may play a role in the tumorigenesis and metastasis of several malignancies including breast cancer, gliomas and metastatic bone disease.

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Mfr.No.	KTE61542
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human C-type mannose receptor 2 (MRC2) ELISA Kit employs a two-site sandwich ELISA to quantitate MRC2.
Alternative Names	MRC2; CD280; CLEC13E; ENDO180; FLJ35911; KIAA0709; UPARAP; endocytic receptor (macrophage mannose receptor family); urokinase plasminogen activator receptor-associated protein
Detection Method	Colorimetric
Kit Components	Human C-type mannose receptor 2 microplate Human C-type mannose receptor 2 standard Human C-type mannose receptor 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human C-type mannose receptor 2 (MRC2) ELISA Kit has high sensitivity and excellent specificity for detection of Human MRC2. No significant cross-reactivity or interference between Human MRC2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MRC2 encodes a member of the mannose receptor family of proteins that contain a fibronectin type II domain and multiple C-type lectin-like domains. C-type mannose receptor 2 plays a role in extracellular matrix remodeling by mediating the internalization and lysosomal degradation of collagen ligands. Expression of MRC2 may play a role in the tumorigenesis and metastasis of several malignancies including breast cancer, gliomas and metastatic bone disease.
Notes	This Human C-type mannose receptor 2 (MRC2) ELISA Kit employs a two-site sandwich ELISA to quantitate MRC2 in samples. An antibody specific for MRC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMRC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	9902

Applications	ELISA
Applications Notes	This Human C-type mannose receptor 2 (MRC2) ELISA Kit employs a two-site sandwich ELISA to quantitate MRC2 in samples. An antibody specific for MRC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMRC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703757	KTE61542	48 T	$339		Add to Cart
BHE13703757	KTE61542	96 T	$589		Add to Cart
BHE13703757	KTE61542	96 Tx5	$2629		Add to Cart
BHE13703757	KTE61542	96 Tx50	$24569		Add to Cart

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