Human C-type mannose receptor 2(MRC2) ELISA kit

Mfr.No.

CSB-EL014784HU

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

mannose receptor, C type 2, CD280, CLEC13E, ENDO180, FLJ35911, KIAA0709, UPARAP, endocytic receptor (macrophage mannose receptor family), urokinase plasminogen activator receptor-associated protein

Recovery

The recovery of pig iNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-100
EDTA plasma (n=4)	93	81-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig iNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	81-95
1:8	Average %	93
	Range %	89-99
1:4	Average %	87
	Range %	83-90
1:8	Average %	89
	Range %	84-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

U/ml	OD1	OD2	Average	Corrected
40	1.818	1.806	1.812	1.666
13.3	1.652	1.635	1.644	1.498
4.44	1.447	1.429	1.438	1.292
1.48	0.987	0.963	0.975	0.829
0.49	0.665	0.648	0.657	0.511
0.16	0.368	0.355	0.362	0.216
0.05	0.249	0.244	0.247	0.101
0	0.145	0.146	0.146	?

Species

Homo sapiens

Sample Type

Cell lysates, Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q9UBG0
Tissue Specificity	Ubiquitous with low expression in brain, placenta, lung, kidney, pancreas, spleen, thymus and colon. Expressed in endothelial cells, fibroblasts and macrophages. Highly expressed in fetal lung and kidney.

Applications	ELISA
Research Area	MRC2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501988	CSB-EL014784HU	96T	$695	Add to Cart
BHE10501988	CSB-EL014784HU	5x96T	$3336	Add to Cart
BHE10501988	CSB-EL014784HU	10x96T	$6602.50	Add to Cart

Human C-type mannose receptor 2(MRC2) ELISA kit

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