Human C-C Motif Chemokine 4

Mfr.No.

E5008Hu

Sensitivity

5.12ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

ACT-2;C-C motif chemokine 4;CCL 4;CCL4;G-26 T-lymphocyte-secreted protein;HC21;LAG-1;Lymphocyte activation gene 1 protein;Macrophage inflammatory protein 1-beta;MIP-1-beta;MIP-1-beta(1-69;MIP-1-beta(3-69;PAT 744;Protein H400;SIS-gamma;Small-inducible cytokine A4;T-cell activation protein 2

Molecular Weight

10212

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E5008Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	388.3	17.95	4.6
Intra-Assay	2	18	809.1	22.89	2.8
Intra-Assay	3	18	182.4	10.68	5.9

Background

Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.

Protocol

These standard curves of E5008Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.134	2.245	2.195
	2.357
600ng/L	1.335	1.36	1.31
	1.385
300ng/L	0.823	0.77	0.72
	0.718
150ng/L	0.473	0.479	0.429
	0.486
75ng/L	0.188	0.198	0.148
	0.209
0ng/L	0.041	0.05	0
	0.059

Gene ID	3883726351
UniProt ID	P13236

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106371	E5008Hu	48T	$320		Add to Cart
BHE12106371	E5008Hu	96T	$458		Add to Cart

Human C-C Motif Chemokine 4 - CCL4 ELISA Kit

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