Human C-C motif chemokine 26(CCL26) ELISA kit

Mfr.No.

CSB-EL004790HU

Alternative Names

chemokine (C-C motif) ligand 26, IMAC, MGC126714, MIP-4a, MIP-4alpha, SCYA26, TSC-1, CC chemokine IMAC, chemokine N1, eotaxin-3, macrophage inflammatory protein 4-alpha, small inducible cytokine A26, small inducible cytokine subfamily A

Reactivity

Human

Detection Range

39 pg/ml - 2500 pg/ml

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse Gzms-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	96-107
EDTA plasma (n=4)	93	88-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse Gzms-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	102
	Range %	95-106
1:2	Average %	86
	Range %	82-92
1:4	Average %	94
	Range %	89-98
1:8	Average %	95
	Range %	89-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.076	2.143	2.110	1.985
400	1.723	1.812	1.768	1.643
200	1.262	1.292	1.277	1.152
100	0.783	0.796	0.790	0.665
50	0.402	0.415	0.409	0.284
25	0.276	0.280	0.278	0.153
12.5	0.191	0.195	0.193	0.068
0	0.124	0.126	0.125	?

Species

Homo sapiens

Sensitivity

9.75 pg/ml

Limit Of Detection

450 nm

UniProt ID	Q9Y258
Antigen	chemokine (C-C motif) ligand 26

Applications

ELISA

Research Area

CCL26

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501709	CSB-EL004790HU	96T	$695	Add to Cart
BHE10501709	CSB-EL004790HU	5x96T	$3336	Add to Cart
BHE10501709	CSB-EL004790HU	10x96T	$6602.50	Add to Cart

Human C-C motif chemokine 26(CCL26) ELISA kit

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