Human C-C Motif Chemokine 25

Mfr.No.

E4392hu

Sensitivity

3.43ng/L

Detection Range

7-1500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human C-C Motif Chemokine 25 (also known as CCL25) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

C-C motif chemokine 25;CCL 25;CCL25;Chemokine TECK;Small-inducible cytokine A25;Thymus-expressed chemokine

Molecular Weight

16609

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E4392hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	480.4	15.21	3.2
Intra-Assay	2	18	113.7	7.27	6.4
Intra-Assay	3	18	295.3	19.27	6.5

Background

Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.

Protocol

These standard curves of E4392hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	2.38	2.358	2.322
	2.335
400ng/L	1.197	1.254	1.218
	1.31
200ng/L	0.827	0.811	0.775
	0.795
100ng/L	0.462	0.445	0.41
	0.429
50ng/L	0.205	0.23	0.194
	0.254
0ng/L	0.039	0.036	0
	0.032

Gene ID	6370
UniProt ID	O15444

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105744	E4392hu	48T	$320		Add to Cart
BHE12105744	E4392hu	96T	$458		Add to Cart

Human C-C Motif Chemokine 25 - CCL25 ELISA Kit

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