Human C-C Chemokine Receptor Type 4

Mfr.No.

E0225Hu

Description

This sandwich kit is for the accurate quantitative detection of human C-C Chemokine Receptor Type 4 (also known as CCR4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

C-C chemokine receptor type 4;C-C CKR-4;CC-CKR-4;CCR 4;CCR4;CCR-4;CD antigen CD194;K5-5

Reactivity

Human

Detection Range

0.05-10ng/ml

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

41403

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.021ng/ml

Background

High affinity receptor for the C-C type chemokines CCL17/TARC, CCL22/MDC and CKLF isoform 1/CKLF1. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival.

Gene ID	1233
UniProt ID	P51679
Antigen	CCR4

Research Area

Others

Protocol

These standard curves of E0225Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
6.4ng/ml	2.354	2.292	2.241
	2.23
3.2ng/ml	1.259	1.249	1.198
	1.238
1.6ng/ml	0.714	0.737	0.687
	0.76
0.8ng/ml	0.416	0.427	0.376
	0.438
0.4ng/ml	0.247	0.276	0.226
	0.305
0ng/ml	0.043	0.05	0
	0.057

Precision

We measured random samples of E0225Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.7	0.15	5.6
Intra-Assay	2	18	3.6	0.1	2.9
Intra-Assay	3	18	1.7	0.1	6.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101941	E0225Hu	96T	$458		Add to Cart
BHE12101941	E0225Hu	48T	$320		Add to Cart

Human C-C Chemokine Receptor Type 4 - CCR4 ELISA Kit

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