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Home / ELISA Kit

Human Brain-specific serine protease 4 (PRSS22) ELISA Kit

SKU : BHE13703324

The deduced 317-amino acid protein contains an N-terminal hydrophobic leader peptide, followed by a propeptide sequence and the serine protease domain, which has the catalytic triad of histidine, aspartic acid, and serine. PRSS22 also has 2 cysteines that form a disulfide bond linking the propeptide and the catalytic domain following proteolytic activation, and it has an N-glycosylation site. PRSS22 lacks a number of residues required by other tryptases to form tetramers. RNA dot blot analysis detected abundant PRSS22 expression in adult esophagus and trachea, with lower levels in adult placenta, pancreas, prostate, and thyroid, and in fetal kidney and lung. There was little to no expression in other tissues examined. Enzymatically active PRSS22 was secreted from a human epithelial cell line and from transfected COS-7 cells.

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Mfr.No.	KTE61108
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Brain-specific serine protease 4 (PRSS22) ELISA Kit employs a two-site sandwich ELISA to quantitate PRSS22.
Alternative Names	PRSS22; BSSP-4; MGC9599; SP001LA; hBSSP-4; brain-specific serine protease 4; prosemin; protease; serine S1 family member 22; tryptase epsilon
Detection Method	Colorimetric
Kit Components	Human Brain-specific serine protease 4 microplate Human Brain-specific serine protease 4 standard Human Brain-specific serine protease 4 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Brain-specific serine protease 4 (PRSS22) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRSS22. No significant cross-reactivity or interference between Human PRSS22 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The deduced 317-amino acid protein contains an N-terminal hydrophobic leader peptide, followed by a propeptide sequence and the serine protease domain, which has the catalytic triad of histidine, aspartic acid, and serine. PRSS22 also has 2 cysteines that form a disulfide bond linking the propeptide and the catalytic domain following proteolytic activation, and it has an N-glycosylation site. PRSS22 lacks a number of residues required by other tryptases to form tetramers. RNA dot blot analysis detected abundant PRSS22 expression in adult esophagus and trachea, with lower levels in adult placenta, pancreas, prostate, and thyroid, and in fetal kidney and lung. There was little to no expression in other tissues examined. Enzymatically active PRSS22 was secreted from a human epithelial cell line and from transfected COS-7 cells.
Notes	This Human Brain-specific serine protease 4 (PRSS22) ELISA Kit employs a two-site sandwich ELISA to quantitate PRSS22 in samples. An antibody specific for PRSS22 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRSS22 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRSS22 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRSS22 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	64063

Applications	ELISA
Applications Notes	This Human Brain-specific serine protease 4 (PRSS22) ELISA Kit employs a two-site sandwich ELISA to quantitate PRSS22 in samples. An antibody specific for PRSS22 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRSS22 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRSS22 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRSS22 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703324	KTE61108	48 T	$339		Add to Cart
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