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Home / ELISA Kit

Human Brain-specific Serine Protease 4 - PRSS22 ELISA Kit

SKU : BHE12115632

Preferentially cleaves the synthetic substrate H-D-Leu-Thr-Arg-pNA compared to tosyl-Gly-Pro-Arg-pNA.

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Bioassay Technology Laboratory

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Mfr.No.

E6564Hu

Sensitivity

0.021ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Brain-specific serine protease 4;BSSP-4;PRSS 22;PRSS22;Serine protease 22;Serine protease 26;Tryptase epsilon

Molecular Weight

33732

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E6564Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	7	0.21	3
Intra-Assay	2	18	3.3	0.19	5.9
Intra-Assay	3	18	1.4	0.08	5.7

Background

Preferentially cleaves the synthetic substrate H-D-Leu-Thr-Arg-pNA compared to tosyl-Gly-Pro-Arg-pNA.

Protocol

These standard curves of E6564Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.515	2.5	2.46
	2.485
6ng/ml	1.32	1.394	1.353
	1.467
3ng/ml	0.902	0.882	0.842
	0.862
1.5ng/ml	0.518	0.477	0.436
	0.436
0.75ng/ml	0.208	0.232	0.192
	0.257
0ng/ml	0.053	0.041	0
	0.028

Gene ID	64063
UniProt ID	Q9GZN4

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115632	E6564Hu	48T	$320		Add to Cart
BHE12115632	E6564Hu	96T	$458		Add to Cart

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