Human Brain derived neurotrophic factor,BDNF ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E04501h

Sensitivity

0.063 ng/mL

Detection Range

0.3125 ng/mL-20 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

brain-derived neurotrophic factor, MGC34632, neurotrophin

Recovery

The recovery of dog IL-12/P70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-96
EDTA plasma (n=4)	97	92-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-12/P70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-105
1:2	Average %	87
	Range %	84-93
1:4	Average %	97
	Range %	91-106
1:8	Average %	94
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sample Type

Serum, Cell culture supeatants, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P23560
Tissue Specificity	Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.

Applications	ELISA
Research Area	BDNF

Storage	Store at 2-8°C. Please refer to protocol.

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