Human Bone morphogenetic protein receptor 1A,BMPR-1A ELISA Kit

Human Bone morphogenetic protein receptor 1A,BMPR-1A ELISA Kit

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Mfr.No.

CSB-E04513h

Alternative Names

bone morphogenetic protein receptor, type IA, 10q23del, ACVRLK3, ALK3, CD292, SKR5, OTTHUMP00000020014, activin A receptor, type II-like kinase 3, serine/threonine-protein kinase receptor R5

Reactivity

Human

Detection Range

0.312 ng/mL-20 ng/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human TXNIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	94-101
EDTA plasma (n=4)	86	82-92

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TXNIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-99
1:2	Average %	97
	Range %	92-102
1:4	Average %	94
	Range %	89-98
1:8	Average %	93
	Range %	87-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.661	2.534	2.598	2.473
500	2.005	2.154	2.080	1.955
250	1.207	1.254	1.231	1.106
125	0.720	0.741	0.731	0.606
62.5	0.405	0.416	0.411	0.286
31.2	0.283	0.288	0.286	0.161
15.6	0.189	0.184	0.187	0.062
0	0.124	0.125	0.125

Species

Homo sapiens

Sensitivity

0.078 ng/mL

Limit Of Detection

450 nm

UniProt ID	P36894
Antigen	bone morphogenetic protein receptor, type IA

Applications

ELISA

Research Area

BMPR1A

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501541	CSB-E04513h	96T	$595	Add to Cart
BHE10501541	CSB-E04513h	5x96T	$2856	Add to Cart
BHE10501541	CSB-E04513h	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.