Human Bone Morphogenetic Protein 7

Mfr.No.

E2245Hu

Sensitivity

0.021ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Human Bone Morphogenetic Protein 7 (also known as BMP7) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

BMP 7;BMP7;BMP-7;Bone morphogenetic protein 7;Eptotermin alfa;OP-1;Osteogenic protein 1

Molecular Weight

49313

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2245Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6.2	0.29	4.6
Intra-Assay	2	18	7.7	0.19	2.5
Intra-Assay	3	18	4	0.15	3.8

Background

Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis. Promotes brown adipocyte differentiation by P38 MAPK pathway-mediated activation of target genes, including members of the SOX family of transcription factors (PubMed: 27923061).

Protocol

These standard curves of E2245Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	1.973	2.064	2.036
	2.155
6ng/ml	1.132	1.139	1.111
	1.146
3ng/ml	0.498	0.455	0.426
	0.411
1.5ng/ml	0.191	0.201	0.173
	0.211
0.75ng/ml	0.111	0.111	0.083
	0.111
0ng/ml	0.029	0.028	0
	0.028

Gene ID	655
UniProt ID	P18075

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103785	E2245Hu	48T	$320		Add to Cart
BHE12103785	E2245Hu	96T	$458		Add to Cart

Human Bone Morphogenetic Protein 7 - BMP7 ELISA Kit

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