Human Bone Morphogenetic Protein 2

Mfr.No.

E1989Hu

Sensitivity

0.01ng/ml

Detection Range

0.05-15ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Human Bone morphogenetic protein 2 (also known as BMP-2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

BMP 2;BMP2;BMP-2;BMP-2A;Bone morphogenetic protein 2;Bone morphogenetic protein 2A

Molecular Weight

44702

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1989Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.4	0.05	3.6
Intra-Assay	2	18	5.9	0.15	2.6
Intra-Assay	3	18	3.7	0.17	4.6

Background

Induces cartilage and bone formation (PubMed: 3201241). Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4 (PubMed: 24362451).

Protocol

These standard curves of E1989Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	2.113	2.035	1.996
	1.957
4ng/ml	1.146	1.1	1.061
	1.055
2ng/ml	0.607	0.609	0.57
	0.611
1ng/ml	0.192	0.231	0.192
	0.27
0.5ng/ml	0.127	0.127	0.088
	0.126
0ng/ml	0.029	0.039	0
	0.049

Gene ID	650
UniProt ID	P12643

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103550	E1989Hu	48T	$320		Add to Cart
BHE12103550	E1989Hu	96T	$458		Add to Cart

Human Bone Morphogenetic Protein 2 - BMP-2 ELISA Kit

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