Matrices listed below were spiked with certain level of BOLA2 and the recovery rates were calculated by comparing the measured value to the expected amount of BOLA2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
83-104
91
EDTA plasma(n=5)
82-101
92
heparin plasma(n=5)
83-103
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BOLA2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
84-101%
82-89%
84-105%
EDTA plasma(n=5)
90-97%
90-101%
90-98%
heparin plasma(n=5)
91-98%
92-100%
92-99%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
Q9H3K6
Applications
ELISA
Applications Notes
BOLA2 ELISA Kit allows for the in vitro quantitative determination of BOLA2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human BOLA2 (BolA-like protein 2 )ELISA Kit