Human BMP8A(Bone Morphogenetic Protein 8A) ELISA Kit

Mfr.No.

ELK5120

Alternative Names

BMP8-A

Reactivity

Human

Detection Range

15.63-1000 pg/mL

Sample Type

Tissue homogenates, Other biological fluids, Serum, Plasma

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant BMP8A and the recovery rates were calculated by comparing the measured value to the expected amount of BMP8A in samples.

Matrix	Recovery range	Average
serum(n=5)	87-97%	90%
EDTA plasma(n=5)	90-105%	97%
Heparin plasma(n=5)	88-102%	95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BMP8A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	95-102%	93-101%	97-105%	95-103%
EDTA plasma(n=5)	93-101%	86-92%	81-93%	79-95%
Heparin plasma(n=5)	95-104%	93-105%	82-101%	91-103%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.103	2.017
500.00	1.586	1.500
250.00	1.130	1.044
125.00	0.837	0.751
62.50	0.570	0.484
31.25	0.361	0.275
15.63	0.213	0.127
0.00	0.086	0.000

Sensitivity

6 pg/mL

Limit Of Detection

1000 pg/mL

UniProt ID	Q7Z5Y6
Antigen	BMP8A
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BMP8A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BMP8A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BMP8A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BMP8A in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Developmental science;Bone metabolism;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15201596	ELK5120	48T	$320		Add to Cart
BHE15201596	ELK5120	96T	$458		Add to Cart

Human BMP8A(Bone Morphogenetic Protein 8A) ELISA Kit

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