Human BMP-5 ELISA Kit PicoKine®

Mfr.No.

EK0310

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.003	0.101	0.143	0.275	0.437	0.889	1.421	2.026

Description

Human BMP-5 ELISA Kit PicoKine® (96 Tests). Quantitate Human BMP5 in cell culture supeatants, serum and bone tissue. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Bone morphogenetic protein 5;BMP-5;BMP5;

Molecular Weight

51737 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A317-H454

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human BMP-5

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Bone tissue homogenate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	242	1573	4363	266	1683	4290
Standard deviation	16.45	69.21	314.13	20.74	82.46	347.49
CV (%)	6.8%	4.4%	7.2%	7.8%	4.9%	8.1%

Protein Function

Induces cartilage and bone formation.

Background

Bone morphogenetic protein 5 is a protein that in humans is encoded by the BMP5 gene.1,2,3 The protein encoded by this gene is member of the TGFbeta superfamily. Bone morphogenetic proteins are known for their ability to induce bone and cartilage development. BMP5 may play a role in certain cancers. Like other BMP's BMP5 is inhibited by chordin and noggin. It is expressed in the trabecular meshwork and optic nerve head and may have a role in the development and normal function. It is also expressed in the lung and liver. BMP-5 is mapped on human chromosome 6.4 The standard used in this kit is recombinant BMP-5 with the monomer molecular mass of 18KDa.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	242	228	246	239	238	6.68	2.8%
Sample 2	1573	1794	1863	1590	1705	126.02	7.3%
Sample 3	4363	4914	4157	4495	4482	276.84	6.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	653
Gene Name	BMP5
UniProt ID	P22003
Protein Name	Bone morphogenetic protein 5
Tissue Specificity	Expressed in the lung and liver.
Sequence	Belongs to the TGF-beta family.

Applications	ELISA
Research Area	TGF Beta

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000347	EK0310	96 wells/kit	$499		Add to Cart

Human BMP-5 ELISA Kit PicoKine®

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