Matrices listed below were spiked with certain level of BMI1 and the recovery rates were calculated by comparing the measured value to the expected amount of BMI1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-102
98
EDTA plasma(n=5)
88-97
92
heparin plasma(n=5)
95-105
100
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BMI1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-94%
85-98%
89-102%
EDTA plasma(n=5)
83-96%
82-97%
85-99%
heparin plasma(n=5)
81-94%
82-98%
81-97%
Sensitivity
< 37.5pg/ml
Notes
For Research Use Only
UniProt ID
P35226
Applications
ELISA
Applications Notes
BMI1 ELISA Kit allows for the in vitro quantitative determination of BMI1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human BMI1(Polycomb complex protein BMI-1) ELISA Kit