Matrices listed below were spiked with certain level of BMF and the recovery rates were calculated by comparing the measured value to the expected amount of BMF in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-104
93
EDTA plasma(n=5)
89-99
92
heparin plasma(n=5)
86-99
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BMF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
BMF ELISA Kit allows for the in vitro quantitative determination of BMF concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human BMF(Bcl-2 Modifying Factor) ELISA Kit