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Home / ELISA Kit

Human Blood group Rh (RHD) ELISA Kit

SKU : BHE13703014

The Rh blood group system is the second most clinically significant of the blood groups, second only to ABO. It is also the most polymorphic of the blood groups, with variations due to deletions, gene conversions, and missense mutations. The Rh blood group includes this gene, which encodes the RhD protein, and a second gene that encodes both the RhC and RhE antigens on a single polypeptide. The two genes, and a third unrelated gene, are found in a cluster on chromosome 1. The classification of Rh-positive and Rh-negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. Multiple transcript variants encoding different isoforms have been found for this gene.

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Mfr.No.	KTE60798
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Blood group Rh (RHD) ELISA Kit employs a two-site sandwich ELISA to quantitate RHD.
Alternative Names	RHD; RP11-335G20.5; CD240D; DIIIc; MGC165007; RH; RH30; RHCED; RHDVA(TT); RHDel; RHPII; RHXIII; Rh4; RhDCw; RhII; RhK562-II; RhPI; D antigen (DCS); RH polypeptide 2; Rh blood group D antigen; Rh blood group
Detection Method	Colorimetric
Kit Components	Human Blood group Rh microplate Human Blood group Rh standard Human Blood group Rh detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Blood group Rh (RHD) ELISA Kit has high sensitivity and excellent specificity for detection of Human RHD. No significant cross-reactivity or interference between Human RHD and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The Rh blood group system is the second most clinically significant of the blood groups, second only to ABO. It is also the most polymorphic of the blood groups, with variations due to deletions, gene conversions, and missense mutations. The Rh blood group includes this gene, which encodes the RhD protein, and a second gene that encodes both the RhC and RhE antigens on a single polypeptide. The two genes, and a third unrelated gene, are found in a cluster on chromosome 1. The classification of Rh-positive and Rh-negative individuals is determined by the presence or absence of the highly immunogenic RhD protein on the surface of erythrocytes. Multiple transcript variants encoding different isoforms have been found for this gene.
Notes	This Human Blood group Rh (RHD) ELISA Kit employs a two-site sandwich ELISA to quantitate RHD in samples. An antibody specific for RHD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRHD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RHD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RHD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6007

Applications	ELISA
Applications Notes	This Human Blood group Rh (RHD) ELISA Kit employs a two-site sandwich ELISA to quantitate RHD in samples. An antibody specific for RHD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRHD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RHD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RHD bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703014	KTE60798	48 T	$339		Add to Cart
BHE13703014	KTE60798	96 T	$589		Add to Cart
BHE13703014	KTE60798	96 Tx5	$2629		Add to Cart
BHE13703014	KTE60798	96 Tx50	$24569		Add to Cart

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