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Home / ELISA Kit

Human Biotinidase(BTD) ELISA kit

SKU : BHE10501519

This Human PENK ELISA Kit was designed for the quantitative measurement of Human PENK protein in serum, plasma, cerebrospinal fluid (CSF). It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

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CUSABIO TECHONOLOGY LLC

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Mfr.No.

CSB-EL002854HU

Reactivity

Human

Sample Type

Serum, Plasma, Tissue Homogenates

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human PENK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-98
EDTA plasma (n=4)		93		87-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PENK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?		Sample		Serum(n=4)
1:1		Average %		86
		Range %		82-92
1:2		Average %		97
		Range %		91-105
1:4		Average %		95
		Range %		90-101
1:8		Average %		107
		Range %		98-112

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.302	2.432	2.367	2.263
500	1.762	1.836	1.799	1.695
250	1.114	1.196	1.155	1.051
125	0.653	0.673	0.663	0.559
61.5	0.375	0.385	0.380	0.276
31.2	0.251	0.271	0.261	0.157
15.6	0.151	0.154	0.153	0.049
0	0.105	0.103	0.104	?

Species

Homo sapiens

Limit Of Detection

450 nm

UniProt ID	P43251
Antigen	Biotinidase

Applications

ELISA

Research Area

BTD

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10501519	CSB-EL002854HU	96T	$695		Add to Cart
BHE10501519	CSB-EL002854HU	5x96T	$3336		Add to Cart
BHE10501519	CSB-EL002854HU	10x96T	$6602.50		Add to Cart

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