Human Biglycan ELISA Kit PicoKine®

Mfr.No.

EK1357

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.007	0.070	0.128	0.240	0.471	0.828	1.465	2.066

Description

Human Biglycan ELISA Kit PicoKine® (96 Tests). Quantitate Human BGN in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Biglycan;Bone/cartilage proteoglycan I;PG-S1;BGN;SLRR1A;

Molecular Weight

41654 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: D38-K368

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Biglycan

Subcellular Localization

Secreted, extracellular space, extracellular matrix .

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	190	939	4176	179	981	4439
Standard deviation	9.12	54.46	167.04	10.2	56.89	217.51
CV (%)	4.8%	5.8%	4%	5.7%	5.8%	4.9%

Protein Function

May be involved in collagen fiber assembly. .

Background

Biglycan (BGN) is a small leucine-rich repeat proteoglycan (SLRP) which is found in a variety of extracellular matrix tissues, including bone, cartilage and tendon. It is an important structural component of articular cartilage and participates in the assembly of the chondrocyte extracellular matrix through formation of protein interactions with type VI collagen and large proteoglycan aggregates. In humans, Biglycan is encoded by the BGN gene. It is mapped to Xq28. Biglycan is believed to play a role in the mineralisation of bone, and its core protein binds to the growth factors BMP-4 and influences its bioactivity. It has been reported that the presence of biglycan is necessary for BMP-4 to exert its effects on osteoblasts. There is also evidence that biglycan can bind to TGF-beta 1. In addition, Biglycan plays an important role in cell signaling.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	190	188	215	183	194	12.38	6.3%
Sample 2	939	960	955	1125	994	75.59	7.6%
Sample 3	4176	4429	4400	3916	4230	206.16	4.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	633
Gene Name	BGN
UniProt ID	P21810
Protein Name	Biglycan
Tissue Specificity	Found in several connective tissues, especially in articular cartilages.
Sequence	Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.

Applications	ELISA
Research Area	Adaptive Immunity,Cardiovascular,Immunology,Innate Immunity,Macrophage/Inflammation,Organelles,Subcellular Markers,T Cells,Tags & Cell Markers

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000951	EK1357	96 wells/kit	$499		Add to Cart

Human Biglycan ELISA Kit PicoKine®

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