Human Beta-defensin 1 - DEFB1 ELISA Kit

Mfr.No.

E1935Hu

Sensitivity

2.31pg/ml

Detection Range

5-2000pg/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Human Beta-defensin 1 (also known as DEFB1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

BD-1;Beta-defensin 1;DEFB 1;DEFB1;Defensin, beta 1;hBD-1

Molecular Weight

7420

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1935Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	153.3	9.66	6.3
Intra-Assay	2	18	236.5	14.17	6
Intra-Assay	3	18	362.7	16.79	4.6

Background

Has bactericidal activity. May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner. Binds to CCR6 and triggers Ca2+ mobilization in the sperm which is important for its motility (PubMed: 25122636).

Protocol

These standard curves of E1935Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200pg/ml	2.312	2.336	2.3
	2.36
600pg/ml	1.732	1.771	1.735
	1.811
300pg/ml	1.202	1.157	1.121
	1.111
150pg/ml	0.618	0.675	0.639
	0.733
75pg/ml	0.42	0.391	0.354
	0.361
0pg/ml	0.03	0.036	0
	0.043

Gene ID	1672
UniProt ID	P60022

Research Area	Microbiology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103499	E1935Hu	48T	$320		Add to Cart
BHE12103499	E1935Hu	96T	$458		Add to Cart

Human Beta-defensin 1 - DEFB1 ELISA Kit

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