Human Beta - Beta-Carotene 9' - 10'-Oxygenase

Mfr.No.

E0019Hu

Sensitivity

0.18ng/ml

Detection Range

0.3-90ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Beta, Beta-carotene 9′,10′-oxygenase (also known as BCO2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

BCDO2;BCO2;B-DIOX-II;Beta,beta-carotene 9',10'-oxygenase;Beta-carotene dioxygenase 2

Molecular Weight

65674

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0019Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	26.73	1.09	4.08
Intra-Assay	2	18	7.99	0.51	6.36
Intra-Assay	3	18	14.88	0.74	4.95

Background

Asymmetrically cleaves beta-carotene at the 9',10' double bond resulting in the formation of beta-apo-10'-carotenal and beta-ionone. Besides beta-carotene, lycopene is also oxidatively cleaved. The apocarotenals formed by this enzyme may be the precursors for the biosynthesis of retinoic acid or exert unknown physiological effects.

Protocol

These standard curves of E0019Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/ml	2.224	2.117	2.076
	2.009
24ng/ml	1.408	1.475	1.434
	1.542
12ng/ml	0.875	0.835	0.794
	0.795
6ng/ml	0.422	0.426	0.385
	0.43
3ng/ml	0.295	0.274	0.233
	0.253
0ng/ml	0.042	0.041	0
	0.04

Gene ID	83875
UniProt ID	Q9BYV7

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101759	E0019Hu	48T	$320		Add to Cart
BHE12101759	E0019Hu	96T	$458		Add to Cart

Human Beta - Beta-Carotene 9' - 10'-Oxygenase - BCO2 ELISA Kit

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