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Home / ELISA Kit

Human Beta-3 adrenergic receptor (ADRB3) ELISA Kit

SKU : BHE13703167

ADRb3 is located mainly in adipose tissue and is involved in the regulation of lipolysis and thermogenesis. Some beta3 agonists have demonstrated antistress effects in animal studies, suggesting it also has a role in the CNS. Beta3-Receptors are found in the gallbladder and in brain adipose tissue. Their role in gallbladder physiology is unknown, but they are thought to play a role in lipolysis and thermogenesis in brown fat.
Beta adrenergic receptors are involved in the epinephrine- and norepinephrine-induced activation of adenylate cyclase through ngono the action of the G proteins of the type Gs.SR 59230A was thought to be a selective beta3 antagonist but later found to also be an antagonist of the alpha1 receptor.

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Mfr.No.	KTE60951
Description	This Human Beta-3 adrenergic receptor (ADRB3) ELISA Kit employs a two-site sandwich ELISA to quantitate ADRB3.
Alternative Names	ADRB3; BETA3AR; FLJ99960;
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Beta-3 adrenergic receptor microplate Human Beta-3 adrenergic receptor standard Human Beta-3 adrenergic receptor detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Beta-3 adrenergic receptor (ADRB3) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADRB3. No significant cross-reactivity or interference between Human ADRB3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	ADRb3 is located mainly in adipose tissue and is involved in the regulation of lipolysis and thermogenesis. Some beta3 agonists have demonstrated antistress effects in animal studies, suggesting it also has a role in the CNS. Beta3-Receptors are found in the gallbladder and in brain adipose tissue. Their role in gallbladder physiology is unknown, but they are thought to play a role in lipolysis and thermogenesis in brown fat. Beta adrenergic receptors are involved in the epinephrine- and norepinephrine-induced activation of adenylate cyclase through ngono the action of the G proteins of the type Gs.SR 59230A was thought to be a selective beta3 antagonist but later found to also be an antagonist of the alpha1 receptor.
Notes	This Human Beta-3 adrenergic receptor (ADRB3) ELISA Kit employs a two-site sandwich ELISA to quantitate ADRB3 in samples. An antibody specific for ADRB3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADRB3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADRB3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADRB3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	155
Antigen	ADRB3
Tag	ADRB3
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Beta-3 adrenergic receptor (ADRB3) ELISA Kit employs a two-site sandwich ELISA to quantitate ADRB3 in samples. An antibody specific for ADRB3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADRB3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADRB3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADRB3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703167	KTE60951	48 T	$339		Add to Cart
BHE13703167	KTE60951	96 T	$589		Add to Cart
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BHE13703167	KTE60951	96 Tx50	$24569		Add to Cart

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