Matrices listed below were spiked with certain level of Beta 2 Transferrrin and the recovery rates were calculated by comparing the measured value to the expected amount of Beta 2 Transferrrin in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-103
96
EDTA plasma(n=5)
85-105
94
heparin plasma(n=5)
89-104
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Beta 2 Transferrrin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
92-98%
87-105%
87-94%
EDTA plasma(n=5)
87-100%
83-98%
82-88%
heparin plasma(n=5)
87-98%
82-96%
81-97%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
Beta 2 Transferrrin Elisa Kit allows for the in vitro quantitative determination of Beta 2 Transferrrin concentrations in serum, plasma, tissue homogenates and other biological fluids.