Human Beta-1 - B4GALT1 ELISA Kit

Mfr.No.

E6542Hu

Alternative Names

B4GALT1;B4GAL-T1;Beta-1,4-galactosyltransferase 1;Beta-1,4-GalTase 1;beta4Gal-T1;Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase;Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase;CDG2D;GGTB2;GT1;GTB;Lactose Synthase A protein;N-acetyllactosamine Synthase;Nal Synthase;Neolactotriaosylceramide beta-1,4-galactosyltransferase;Processed beta-1,4-galactosyltransferase 1);UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1;UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1

Reactivity

Human

Detection Range

0.05-20ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

43920

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.023ng/ml

Background

The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.

Gene ID	2683
UniProt ID	P15291
Antigen	B4GALT1

Research Area

Signal Transduction; Developmental Biology

Protocol

These standard curves of E6542Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.025	2.016	1.976
	2.007
6ng/ml	1.322	1.322	1.282
	1.323
3ng/ml	0.673	0.664	0.624
	0.655
1.5ng/ml	0.321	0.312	0.272
	0.303
0.75ng/ml	0.182	0.18	0.14
	0.178
0ng/ml	0.052	0.04	0
	0.028

Precision

We measured random samples of E6542Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.31	0.21	4.86
Intra-Assay	2	18	7.03	0.26	3.66
Intra-Assay	3	18	2.98	0.15	5.02

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115376	E6542Hu	48T	$320		Add to Cart
BHE12115376	E6542Hu	96T	$458		Add to Cart

Human Beta-1 - B4GALT1 ELISA Kit

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