Human Beta-1,4-galactosyltransferase 1(B4GALT1) ELISA kit

Human Beta-1,4-galactosyltransferase 1(B4GALT1) ELISA kit

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Mfr.No.

CSB-EL002513HU

Alternative Names

UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1, B4GAL-T1, DKFZp686N19253, GGTB2, GT1, GTB, MGC50983, beta4Gal-T1, beta-1,4-galactosyltransferase 1, glycoprotein-4-beta-galactosyltransferase 2, lactose synthase

Reactivity

Human

Detection Range

0.156 ng/mL-10 ng/mL

Sample Type

Cell lysates, Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of bovine TTR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-104
EDTA plasma (n=4)	89	84-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine TTR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	95
	Range %	91-97
1:200	Average %	103
	Range %	99-107
1:400	Average %	98
	Range %	93-103
1:800	Average %	92
	Range %	87-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
5000	0.147	0.153	0.150
2500	0.266	0.275	0.271
1250	0.511	0.512	0.512
625	0.905	0.903	0.904
312.5	1.674	1.787	1.731
0	2.689	2.599	2.644

Species

Homo sapiens

Sensitivity

0.039 ng/mL

Limit Of Detection

450 nm

UniProt ID	P15291
Antigen	UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1

Applications

ELISA

Research Area

B4GALT1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501485	CSB-EL002513HU	96T	$595	Add to Cart
BHE10501485	CSB-EL002513HU	5x96T	$2856	Add to Cart
BHE10501485	CSB-EL002513HU	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.