Matrices listed below were spiked with certain level of BDNF and the recovery rates were calculated by comparing the measured value to the expected amount of BDNF in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-101
96
EDTA plasma(n=5)
86-102
94
heparin plasma(n=5)
86-101
90
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BDNF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
BDNF ELISA Kit allows for the in vitro quantitative determination of BDNF concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human BDNF(Brain Derived Neurotrophic Factor) ELISA Kit