Human Bcl2 Antagonist of Cell Death

Mfr.No.

E4102hu

Description

This sandwich kit is for the accurate quantitative detection of human Bcl2 Antagonist of Cell Death (also known as BAD) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

BAD;Bcl2 antagonist of cell death;Bcl2-associated agonist of cell death;Bcl-2-binding component 6;Bcl2-L-8;Bcl-2-like protein 8;Bcl-xL/Bcl-2-associated death promoter

Reactivity

Human

Detection Range

5-1800ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

18392

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

2.42ng/L

Background

Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Gene ID	572
UniProt ID	Q92934
Antigen	BAD

Research Area

Cancer

Protocol

These standard curves of E4102hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1000ng/L	1.995	1.875	1.826
	1.755
500ng/L	0.951	0.918	0.869
	0.885
250ng/L	0.548	0.49	0.441
	0.432
125ng/L	0.24	0.245	0.196
	0.25
62.5ng/L	0.067	0.105	0.057
	0.144
0ng/L	0.052	0.049	0
	0.046

Precision

We measured random samples of E4102hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	602.8	25.41	4.2
Intra-Assay	2	18	795.6	22.8	2.9
Intra-Assay	3	18	248.9	11.95	4.8

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105508	E4102hu	48T	$320		Add to Cart
BHE12105508	E4102hu	96T	$458		Add to Cart

Human Bcl2 Antagonist of Cell Death - BAD ELISA Kit

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