Human Bcl-2-like protein 11(BCL2L11) ELISA kit

Human Bcl-2-like protein 11(BCL2L11) ELISA kit

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Mfr.No.

CSB-EL002615HU

Alternative Names

BCL2-like 11 (apoptosis facilitator), BAM, BIM, BIM-alpha6, BIM-beta6, BIM-beta7, BOD, BimEL, BimL, BCL2-like 11, OTTHUMP00000197013, bcl-2 interacting mediator of cell death, bcl-2 interacting protein Bim, bcl-2-related ovarian death agoni

Reactivity

Human

Detection Range

23.44 pg/mL-1500 pg/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-101
EDTA plasma (n=4)	89	82-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	82-88
1:2	Average %	94
	Range %	89-98
1:4	Average %	104
	Range %	97-109
1:8	Average %	99
	Range %	93-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
12	2.389	2.501	2.445	2.281
6	1.445	1.529	1.487	1.323
3	0.905	0.984	0.945	0.781
1.5	0.656	0.633	0.645	0.481
0.75	0.345	0.349	0.347	0.183
0.37	0.287	0.265	0.276	0.112
0.18	0.223	0.218	0.221	0.057
0	0.159	0.169	0.164

Species

Homo sapiens

Sensitivity

5.86 pg/mL

Limit Of Detection

450 nm

UniProt ID	O43521
Antigen	BCL2-like 11 (apoptosis facilitator)

Applications

ELISA

Research Area

BCL2L11

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501482	CSB-EL002615HU	96T	$695	Add to Cart
BHE10501482	CSB-EL002615HU	5x96T	$3336	Add to Cart
BHE10501482	CSB-EL002615HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.