Human BCKDHB(2-Oxoisovalerate Dehydrogenase Subunit Beta, Mitochondrial) ELISA Kit

Mfr.No.

EH6661

Description

Human BCKDHB(2-Oxoisovalerate Dehydrogenase Subunit Beta, Mitochondrial) ELISA Kit

Alternative Names

2-oxoisovalerate dehydrogenase subunit beta, mitochondrial ELISA Kit, Branched-chain alpha-keto acid dehydrogenase E1 component beta chain ELISA Kit, BCKDE1B ELISA Kit, BCKDH E1-beta ELISA Kit, BCKDHB ELISA Kit

Reactivity

Human

Detection Method

Double Antibody

Detection Range

78.125-5000pg/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of BCKDHB and the recovery rates were calculated by comparing the measured value to the expected amount of BCKDHB in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	87-105	95
EDTA plasma(n=5)	88-100	95
heparin plasma(n=5)	87-102	93

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BCKDHB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	90-105%	87-105%	85-102%
EDTA plasma(n=5)	87-97%	88-96%	83-97%
heparin plasma(n=5)	84-99%	87-93%	82-100%

Sensitivity

< 46.875pg/ml

Notes

For Research Use Only

UniProt ID	P21953

Applications	ELISA
Applications Notes	BCKDHB ELISA Kit allows for the in vitro quantitative determination of BCKDHB concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10807977	EH6661	96T	$400		Add to Cart

Human BCKDHB(2-Oxoisovalerate Dehydrogenase Subunit Beta, Mitochondrial) ELISA Kit

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