Human BCHE/Butyrylcholinesterase ELISA Kit PicoKine®

Mfr.No.

EK1507

Sensitivity

<10 pg/ml

Detection Range

625 pg/ml - 40,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	625	1250	2500	5000	10000	20000	40000
O.D.	0.015	0.076	0.144	0.250	0.466	0.985	1.607	2.036

Description

Human BCHE/Butyrylcholinesterase ELISA Kit PicoKine® (96 Tests). Quantitate Human BCHE in cell culture supeatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Cholinesterase;3.1.1.8;Acylcholine acylhydrolase;Butyrylcholine esterase;Choline esterase II;Pseudocholinesterase;BCHE;CHE1;

Molecular Weight

68418 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E29-L602

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human BCHE

Subcellular Localization

Secreted .

Sample Type

Cell culture supeatants, Serum, Plasma, Saliva, Urine, Human milk

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1205	4078	24826	1297	4376	24447
Standard deviation	66.27	322.16	1613.69	80.41	380.71	1662.39
CV (%)	5.5%	7.9%	6.5%	6.2%	8.7%	6.8%

Protein Function

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters. .

Background

Butyrylcholinesterase is a nonspecific cholinesterase enzyme that in humans is encoded by the BCHE gene. This gene is mapped to 3q26. It is a member of the type-B carboxylesterase/lipase family of proteins. The encoded enzyme exhibits broad substrate specificity and is involved in the detoxification of poisons including organophosphate nerve agents and pesticides, and the metabolism of drugs including cocaine, heroin and aspirin. Humans homozygous for certain mutations in this gene exhibit prolonged apnea after administration of the muscle relaxant succinylcholine.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1205	1122	1225	1228	1195	43.06	3.6%
Sample 2	4078	4285	4341	4276	4245	99.58	2.3%
Sample 3	24826	24993	26084	24591	25123	572.63	2.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	590
Gene Name	BCHE
UniProt ID	P06276
Protein Name	Cholinesterase
Tissue Specificity	Detected in blood plasma (at protein level). Present in most cells except erythrocytes. .

Applications	ELISA
Research Area	Alzheimer's Disease,Drug Metabolism,Metabolic Signaling Pathways,Metabolism,Neurodegenerative Disease,Neurology Process,Neuroscience,Neurotransmitter,Pathways and Processes,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001277	EK1507	96 wells/kit	$499		Add to Cart

Human BCHE/Butyrylcholinesterase ELISA Kit PicoKine®

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