Matrices listed below were spiked with certain level of B7H6 and the recovery rates were calculated by comparing the measured value to the expected amount of B7H6 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-103
98
EDTA plasma(n=5)
93-103
99
heparin plasma(n=5)
86-104
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of B7H6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
B7H6 ELISA Kit allows for the in vitro quantitative determination of B7H6 concentrations in serum, plasma, tissue homogenates and other biological fluids.