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Home / ELISA Kit

Human B1 bradykinin receptor(BDKRB1) ELISA kit

SKU : BHE10501460

This Human PDGFC ELISA Kit was designed for the quantitative measurement of Human PDGFC protein in serum, urine, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

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CUSABIO TECHONOLOGY LLC

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Mfr.No.

CSB-EL002651HU

Sensitivity

6.25 pg/mL

Detection Range

25 pg/mL-1600 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

bradykinin receptor B1, B1BKR, B1R, BKB1R, BKR1, BRADYB1, BK-1 receptor, bradykinin B1 receptor, bradykinin receptor 1

Recovery

The recovery of human VEGF-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type		Average % Recovery		Range
Serum (n=5)		91		83-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human VEGF-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?		Sample		Serum(n=4)
1:1		Average %		85
		Range %		80-90
1:2		Average %		96
		Range %		90-100
1:4		Average %		98
		Range %		91-105
1:8		Average %		90
		Range %		82-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.806	2.712	2.759	2.659
200	2.012	2.066	2.039	1.939
100	1.473	1.384	1.429	1.329
50	0.958	0.893	0.926	0.826
25	0.494	0.473	0.484	0.384
12.5	0.321	0.301	0.311	0.211
6.25	0.254	0.249	0.252	0.152
0	0.102	0.097	0.100	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Protocol

UniProt ID	P46663

Applications	ELISA
Research Area	BDKRB1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10501460	CSB-EL002651HU	96T	$695		Add to Cart
BHE10501460	CSB-EL002651HU	5x96T	$3336		Add to Cart
BHE10501460	CSB-EL002651HU	10x96T	$6602.50		Add to Cart

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