Human B-cell leukemia/lymphoma 2,Bcl-2 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08853h

Sensitivity

0.225 ng/mL

Detection Range

0.9 ng/mL-60 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

B-cell CLL/lymphoma 2, Bcl-2, B-cell lymphoma protein 2, OTTHUMP00000163680

Recovery

The recovery of human TUBB3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	98	96-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TUBB3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	94
	Range %	88-99
1:2	Average %	99
	Range %	93-105
1:4	Average %	86
	Range %	81-93
1:8	Average %	90
	Range %	84-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P10415

Applications	ELISA
Research Area	BCL2

Storage	Store at 2-8°C. Please refer to protocol.

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