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Home / ELISA Kit

Human B cell activation factor (BAFF) ELISA Kit

SKU : BHE13702423

B-cell activating factor (BAFF) is the tumor necrosis factor ligand superfamily member 13B (TNFLSF13B), also known as B Lymphocyte Stimulator (BLyS) and TNF- and APOL-related leukocyte expressed ligand (TALL-1) and the Dendritic cell-derived TNF-like molecule (CD257 antigen; cluster of differentiation 257). BAFF is a 285-amino acid long peptide glycoprotein which undergoes glycosylation at residue 124. It is expressed as transmembrane protein on various cell types including monocytes, dendritic cells and bone marrow stromal cells. The transmembrane form can be cleaved from the membrane, generating a soluble protein fragment. BAFF is the natural ligand of three unusual tumor necrosis factor receptors named BAFF-R, TACI, and BCMA, all of which have differing binding affinities for it.

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Mfr.No.	KTE60205
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human B cell activation factor (BAFF) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF13B.
Alternative Names	TNFSF13B; BAFF; BLYS; CD257; DTL; TALL-1; TALL1; THANK; TNFSF20; ZTNF4; ApoL related ligand TALL-1; B-cell activating factor; B-lymphocyte stimulator; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolo
Detection Method	Colorimetric
Kit Components	Human B cell activation factor microplate Human B cell activation factor standard Human B cell activation factor detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human B cell activation factor (BAFF) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNFSF13B. No significant cross-reactivity or interference between Human TNFSF13B and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	B-cell activating factor (BAFF) is the tumor necrosis factor ligand superfamily member 13B (TNFLSF13B), also known as B Lymphocyte Stimulator (BLyS) and TNF- and APOL-related leukocyte expressed ligand (TALL-1) and the Dendritic cell-derived TNF-like molecule (CD257 antigen; cluster of differentiation 257). BAFF is a 285-amino acid long peptide glycoprotein which undergoes glycosylation at residue 124. It is expressed as transmembrane protein on various cell types including monocytes, dendritic cells and bone marrow stromal cells. The transmembrane form can be cleaved from the membrane, generating a soluble protein fragment. BAFF is the natural ligand of three unusual tumor necrosis factor receptors named BAFF-R, TACI, and BCMA, all of which have differing binding affinities for it.
Notes	This Human B cell activation factor (BAFF) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF13B in samples. An antibody specific for TNFSF13B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFSF13B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFSF13B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFSF13B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	10673

Applications	ELISA
Applications Notes	This Human B cell activation factor (BAFF) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF13B in samples. An antibody specific for TNFSF13B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFSF13B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFSF13B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFSF13B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702423	KTE60205	48 T	$359		Add to Cart
BHE13702423	KTE60205	96 T	$619		Add to Cart
BHE13702423	KTE60205	96 Tx5	$2809		Add to Cart
BHE13702423	KTE60205	96 Tx50	$26209		Add to Cart

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