Matrices listed below were spiked with certain level of ATR and the recovery rates were calculated by comparing the measured value to the expected amount of ATR in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-105
97
EDTA plasma(n=5)
87-105
95
heparin plasma(n=5)
85-100
90
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ATR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-104%
86-105%
85-101%
EDTA plasma(n=5)
90-98%
89-98%
85-101%
heparin plasma(n=5)
81-100%
83-95%
86-99%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
Q13535
Applications
ELISA
Applications Notes
ATR ELISA Kit allows for the in vitro quantitative determination of ATR concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human ATR(Serine/threonine-protein kinase ATR) ELISA Kit