Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of ATPB and the recovery rates were calculated by comparing the measured value to the expected amount of ATPB in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-103
94
EDTA plasma(n=5)
88-102
92
heparin plasma(n=5)
85-99
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ATPB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-102%
87-100%
96-103%
EDTA plasma(n=5)
82-101%
82-98%
82-97%
heparin plasma(n=5)
86-98%
83-100%
82-99%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
P06576
Antigen
ATPB
Applications
ELISA
Applications Notes
ATPB ELISA Kit allows for the in vitro quantitative determination of ATPB concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human ATPB (ATP synthase subunit beta) ELISA Kit