Human ATP synthase lipid-binding protein, mitochondrial(ATP5G1) ELISA kit

Mfr.No.

CSB-EL002359HU

Sensitivity

5.86 pg/mL

Detection Range

23.5 pg/mL-1500 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C1 (subunit 9), ATP5A, ATP5G, ATP synthase lipid-binding protein, mitochondrial, ATP synthase proteolipid P1, ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C1, ATP synthase, H+ transporting, mitocho

Recovery

The recovery of rat PDGF-AB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	83-90
EDTA plasma (n=4)	102	97-106

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-AB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-99
1:2	Average %	88
	Range %	84-93
1:4	Average %	105
	Range %	101-109
1:8	Average %	100
	Range %	95-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	1.978	2.012	1.995	1.827
250	1.568	1.601	1.585	1.417
125	1.016	1.032	1.024	0.856
62.5	0.527	0.518	0.523	0.355
31.2	0.354	0.361	0.358	0.190
15.6	0.257	0.248	0.253	0.085
7.8	0.204	0.211	0.208	0.040
0	0.166	0.169	0.168	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P05496

Applications	ELISA
Research Area	ATP5G1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10501452	CSB-EL002359HU	96T	$695	Add to Cart
BHE10501452	CSB-EL002359HU	5x96T	$3336	Add to Cart
BHE10501452	CSB-EL002359HU	10x96T	$6602.50	Add to Cart

Human ATP synthase lipid-binding protein, mitochondrial(ATP5G1) ELISA kit

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