Human Atp-citrate Synthase

Mfr.No.

E6527Hu

Alternative Names

ACL;ACLY;ATP-citrate;ATP-citrate Synthase;ATPCL;Citrate cleavage enzyme;CLATP;pro-S--lyase

Reactivity

Human

Detection Range

20-6000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

120839

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.75ng/L

Background

Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate for de novo cholesterol and fatty acid synthesis.

Gene ID	47
UniProt ID	P53396
Antigen	ACLY

Research Area

Signal Transduction; Cancer; Metabolism

Protocol

These standard curves of E6527Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/L	2.221	2.157	2.116
	2.093
1600ng/L	1.467	1.512	1.471
	1.557
800ng/L	0.576	0.591	0.549
	0.605
400ng/L	0.445	0.374	0.333
	0.303
200ng/L	0.131	0.166	0.124
	0.2
0ng/L	0.027	0.041	0
	0.056

Precision

We measured random samples of E6527Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	908.44	30.18	3.32
Intra-Assay	2	18	1487.99	45.85	3.08
Intra-Assay	3	18	636.06	26.92	4.23

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115387	E6527Hu	48T	$320		Add to Cart
BHE12115387	E6527Hu	96T	$458		Add to Cart

Human Atp-citrate Synthase - ACLY ELISA Kit

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