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Home / ELISA Kit

Human ATP-binding cassette transporter A1 (ABCA1) ELISA Kit

SKU : BHE13703063

ABCA1 was discovered as the mutation causing Tangier's Disease by several groups in 1998. Gerd Schmitz's group in Germany and Michael Hayden's group in British Columbia were using standard genetics techniques and DNA from family pedigrees to locate the mutation.
Richard Lawn's group at CV Therapeutics in Palo Alto, CA used cDNA microarrays, which were relatively new at the time, to assess gene expression profiles from cell lines created from normal and effected individuals. They showed cell lines from patients with Tangier's disease showed differential regulation of the ABCA1 gene. Subsequent sequencing of the gene identified the mutations. This group received an award from the American Heart Association for their discovery.

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Mfr.No.	KTE60847
Description	This Human ATP-binding cassette transporter A1 (ABCA1) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCA1.
Alternative Names	ABCA1; ABC-1; ABC1; CERP; FLJ14958; HDLDT1; MGC164864; MGC165011; TGD; ATP binding cassette transporter 1; ATP-binding cassette 1; ATP-binding cassette transporter A1; ATP-binding cassette; sub-family A membe
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human ATP-binding cassette transporter A1 microplate Human ATP-binding cassette transporter A1 standard Human ATP-binding cassette transporter A1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human ATP-binding cassette transporter A1 (ABCA1) ELISA Kit has high sensitivity and excellent specificity for detection of Human ABCA1. No significant cross-reactivity or interference between Human ABCA1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	ABCA1 was discovered as the mutation causing Tangier's Disease by several groups in 1998. Gerd Schmitz's group in Germany and Michael Hayden's group in British Columbia were using standard genetics techniques and DNA from family pedigrees to locate the mutation. Richard Lawn's group at CV Therapeutics in Palo Alto, CA used cDNA microarrays, which were relatively new at the time, to assess gene expression profiles from cell lines created from normal and effected individuals. They showed cell lines from patients with Tangier's disease showed differential regulation of the ABCA1 gene. Subsequent sequencing of the gene identified the mutations. This group received an award from the American Heart Association for their discovery.
Notes	This Human ATP-binding cassette transporter A1 (ABCA1) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCA1 in samples. An antibody specific for ABCA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	19
Antigen	ABCA1
Tag	ABCA1
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human ATP-binding cassette transporter A1 (ABCA1) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCA1 in samples. An antibody specific for ABCA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703063	KTE60847	48 T	$359		Add to Cart
BHE13703063	KTE60847	96 T	$619		Add to Cart
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