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Home / ELISA Kit

Human ATP-binding cassette sub-family C member 11 (ABCC11) ELISA Kit

SKU : BHE13703072

ABCC11 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This ABC full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. The product of this gene participates in physiological processes involving bile acids, conjugated steroids, and cyclic nucleotides. In addition, an SNP in this gene is responsible for determination of human earwax type. This gene and family member ABCC12 are determined to be derived by duplication and are both localized to chromosome 16q12.1. Multiple alternatively spliced transcript variants have been described for this gene.

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Mfr.No.	KTE60856
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human ATP-binding cassette sub-family C member 11 (ABCC11) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCC11.
Alternative Names	ABCC11; EWWD; MRP8; WW; ATP-binding cassette protein C11; ATP-binding cassette transporter MRP8; ATP-binding cassette; sub-family C; member 11; OTTHUMP00000164191; multi-resistance protein 8
Detection Method	Colorimetric
Kit Components	Human ATP-binding cassette sub-family C member 11 microplate Human ATP-binding cassette sub-family C member 11 standard Human ATP-binding cassette sub-family C member 11 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human ATP-binding cassette sub-family C member 11 (ABCC11) ELISA Kit has high sensitivity and excellent specificity for detection of Human ABCC11. No significant cross-reactivity or interference between Human ABCC11 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	ABCC11 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This ABC full transporter is a member of the MRP subfamily which is involved in multi-drug resistance. The product of this gene participates in physiological processes involving bile acids, conjugated steroids, and cyclic nucleotides. In addition, an SNP in this gene is responsible for determination of human earwax type. This gene and family member ABCC12 are determined to be derived by duplication and are both localized to chromosome 16q12.1. Multiple alternatively spliced transcript variants have been described for this gene.
Notes	This Human ATP-binding cassette sub-family C member 11 (ABCC11) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCC11 in samples. An antibody specific for ABCC11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCC11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCC11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCC11 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	85320

Applications	ELISA
Applications Notes	This Human ATP-binding cassette sub-family C member 11 (ABCC11) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCC11 in samples. An antibody specific for ABCC11 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCC11 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCC11 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCC11 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703072	KTE60856	48 T	$339		Add to Cart
BHE13703072	KTE60856	96 T	$589		Add to Cart
BHE13703072	KTE60856	96 Tx5	$2629		Add to Cart
BHE13703072	KTE60856	96 Tx50	$24569		Add to Cart

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