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Home / ELISA Kit

Human ATP-binding cassette sub-family B member 5 (ABCB5) ELISA Kit

SKU : BHE13703069

ABCB5 (ATP-Binding Cassette Subfamily B (MDR/TAP) Member 5) is a human ABC transporter and P-glycoprotein family member principally expressed in physiological skin and human malignant melanoma.ABCB5-alpha and -beta diverge after exon 6, with ABCB5-alpha including a seventh exon that encodes its 3-prime UTR, and ABCB5-beta including 14 more exons. The 131-amino acid ABCB5-alpha protein has a calculated molecular mass of 15 kD. It has an ABC signature motif and a Walker B consensus sequence, but no Walker A consensus sequence. In contrast, ABCB5-beta has an N-terminal ABC signature motif and Walker B motif, followed by 6 transmembrane domains and C-terminal Walker A, ABC signature, and Walker B motifs.

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Mfr.No.	KTE60853
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human ATP-binding cassette sub-family B member 5 (ABCB5) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCB5.
Alternative Names	ABCB5; ABCB5alpha; ABCB5beta; EST422562; ATP-binding cassette protein; ATP-binding cassette; sub-family B; member 5; P-glycoprotein ABCB5
Detection Method	Colorimetric
Kit Components	Human ATP-binding cassette sub-family B member 5 microplate Human ATP-binding cassette sub-family B member 5 standard Human ATP-binding cassette sub-family B member 5 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human ATP-binding cassette sub-family B member 5 (ABCB5) ELISA Kit has high sensitivity and excellent specificity for detection of Human ABCB5. No significant cross-reactivity or interference between Human ABCB5 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	ABCB5 (ATP-Binding Cassette Subfamily B (MDR/TAP) Member 5) is a human ABC transporter and P-glycoprotein family member principally expressed in physiological skin and human malignant melanoma.ABCB5-alpha and -beta diverge after exon 6, with ABCB5-alpha including a seventh exon that encodes its 3-prime UTR, and ABCB5-beta including 14 more exons. The 131-amino acid ABCB5-alpha protein has a calculated molecular mass of 15 kD. It has an ABC signature motif and a Walker B consensus sequence, but no Walker A consensus sequence. In contrast, ABCB5-beta has an N-terminal ABC signature motif and Walker B motif, followed by 6 transmembrane domains and C-terminal Walker A, ABC signature, and Walker B motifs.
Notes	This Human ATP-binding cassette sub-family B member 5 (ABCB5) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCB5 in samples. An antibody specific for ABCB5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCB5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCB5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCB5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	340273

Applications	ELISA
Applications Notes	This Human ATP-binding cassette sub-family B member 5 (ABCB5) ELISA Kit employs a two-site sandwich ELISA to quantitate ABCB5 in samples. An antibody specific for ABCB5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyABCB5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ABCB5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ABCB5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703069	KTE60853	48 T	$339		Add to Cart
BHE13703069	KTE60853	96 T	$589		Add to Cart
BHE13703069	KTE60853	96 Tx5	$2629		Add to Cart
BHE13703069	KTE60853	96 Tx50	$24569		Add to Cart

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