Matrices listed below were spiked with certain level of ATF-6 and the recovery rates were calculated by comparing the measured value to the expected amount of ATF-6 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-102
97
EDTA plasma(n=5)
87-99
95
heparin plasma(n=5)
89-102
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ATF-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
ATF-6 ELISA Kit allows for the in vitro quantitative determination of ATF-6 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human ATF-6(Activating Transcription Factor 6) ELISA Kit