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Home / ELISA Kit

Human Asparaginyl-tRNA synthetase, cytoplasmic (NARS) ELISA Kit

SKU : BHE13703590

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Asparaginyl-tRNA synthetase is localized to the cytoplasm and belongs to the class II family of tRNA synthetases. The N-terminal domain represents the signature sequence for the eukaryotic asparaginyl-tRNA synthetases.

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Mfr.No.	KTE61375
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Asparaginyl-tRNA synthetase, cytoplasmic (NARS) ELISA Kit employs a two-site sandwich ELISA to quantitate NARS.
Alternative Names	NARS; ASNRS; NARS1; asparagine tRNA ligase 1; cytoplasmic
Detection Method	Colorimetric
Kit Components	Human Asparaginyl-tRNA synthetase, cytoplasmic microplate Human Asparaginyl-tRNA synthetase, cytoplasmic standard Human Asparaginyl-tRNA synthetase, cytoplasmic detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Asparaginyl-tRNA synthetase, cytoplasmic (NARS) ELISA Kit has high sensitivity and excellent specificity for detection of Human NARS. No significant cross-reactivity or interference between Human NARS and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Asparaginyl-tRNA synthetase is localized to the cytoplasm and belongs to the class II family of tRNA synthetases. The N-terminal domain represents the signature sequence for the eukaryotic asparaginyl-tRNA synthetases.
Notes	This Human Asparaginyl-tRNA synthetase, cytoplasmic (NARS) ELISA Kit employs a two-site sandwich ELISA to quantitate NARS in samples. An antibody specific for NARS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNARS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NARS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NARS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	4677

Applications	ELISA
Applications Notes	This Human Asparaginyl-tRNA synthetase, cytoplasmic (NARS) ELISA Kit employs a two-site sandwich ELISA to quantitate NARS in samples. An antibody specific for NARS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNARS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NARS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NARS bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703590	KTE61375	48 T	$339		Add to Cart
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