ASIP ELISA Kit, Agouti-signaling protein ELISA Kit, Agouti switch protein ELISA Kit, ASP ELISA Kit, AGTI ELISA Kit, AGTIL ELISA Kit
Reactivity
Human
Detection Method
Double Antibody
Detection Range
1.563-100pg/ml
Sample Type
Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of ASIP and the recovery rates were calculated by comparing the measured value to the expected amount of ASIP in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-95
91
EDTA plasma(n=5)
88-105
96
heparin plasma(n=5)
86-97
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ASIP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-103%
89-105%
85-102%
EDTA plasma(n=5)
84-94%
82-96%
82-95%
heparin plasma(n=5)
86-100%
84-100%
86-90%
Sensitivity
< 0.938pg/ml
Notes
For Research Use Only
UniProt ID
P42127
Applications
ELISA
Applications Notes
ASIP ELISA Kit allows for the in vitro quantitative determination of ASIP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human ASIP (Agouti-signaling protein) ELISA Kit