Human Aquaporin 1,AQP-1 ELISA Kit

Human Aquaporin 1,AQP-1 ELISA Kit

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Mfr.No.

CSB-E08248h

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

aquaporin 1 (Colton blood group), AQP-CHIP, CHIP28, CO, MGC26324, Colton blood group, aquaporin 1, aquaporin 1 (channel-forming integral protein, 28kDa, CO blood group), channel-like integral membrane protein, 28-kDa

Recovery

The recovery of rat FDP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	105	99-109

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat FDP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	97
	Range %	93-102
1:2	Average %	90
	Range %	85-94
1:4	Average %	95
	Range %	91-99
1:8	Average %	85
	Range %	81-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.305	2.251	2.278	2.091
500	1.711	1.641	1.676	1.489
250	1.203	1.221	1.212	1.025
125	0.793	0.815	0.804	0.617
62.5	0.529	0.566	0.548	0.361
31.25	0.467	0.441	0.454	0.267
15.6	0.264	0.247	0.256	0.069
0	0.193	0.181	0.187	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P29972

Applications	ELISA
Research Area	AQP1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10501417	CSB-E08248h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.