Matrices listed below were spiked with certain level of AQP12A and the recovery rates were calculated by comparing the measured value to the expected amount of AQP12A in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
94-99
97
EDTA plasma(n=5)
92-103
99
heparin plasma(n=5)
88-101
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AQP12A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
AQP12A ELISA Kit allows for the in vitro quantitative determination of AQP12A concentrations in serum, plasma, tissue homogenates and other biological fluids.